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Genetics Faculty

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Ann Harris
Professor/Vice Chair of Research
Department of Genetics
School of Medicine
Case Western Reserve University
Biomedical Research Building 724
2109 Adelbert Road
Cleveland, Ohio 44106-4955
Tel: (216) 368-6651
Fax: (216) 368-3432
E-mail: ann.harris@case.edu



About Ann Harris


Research

The main research focus in the laboratory is on transcriptional networks that coordinate chromatin architecture and gene expression in epithelial cells lining the human lung, intestine, pancreas and male genital ducts. These sites are all involved in the common human genetic disease cystic fibrosis (CF). The respiratory epithelium is also compromised in chronic obstructive pulmonary disease (COPD) and asthma. The digestive tract epithelium is a site of active pathology in inflammatory bowel disease (IBD) and other related disorders. Obstruction of the male genital ducts underlies several diseases resulting in male infertility, such as congenital bilateral absence of the vas deferens (CBAVD). Moreover, epithelial cells are the sites of initiation of many types of cancer in the lung, pancreas and intestine. Hence, our work is pertinent to many diseases of epithelia and our research goals are to facilitate new therapeutics for these conditions.


Selected Publications

Screening for regulatory variants in 460kb encompassing the CFTR locus in cystic fibrosis patients.
Kerschner JL, Ghosh S, Paranjapye A, Cosme WR, Audrézet MP, Nakakuki M, Ishiguro H, Férec C, Rommens J, Harris A
J Mol Diagn (2018);:
See PubMed abstract

A sheep model of cystic fibrosis generated by CRISPR/Cas9 disruption of the CFTR gene.
Fan Z, Perisse IV, Cotton CU, Regouski M, Meng Q, Domb C, Van Wettere AJ, Wang Z, Harris A, White KL, Polejaeva IA
JCI Insight (2018);3(19):
See PubMed abstract

Region-specific innate antiviral responses of the human epididymis.
Browne JA, Leir SH, Eggener SE, Harris A
Mol Cell Endocrinol (2018);473:72-78
See PubMed abstract

A novel transcriptional network for the androgen receptor in human epididymis epithelial cells.
Yang R, Browne JA, Eggener SE, Leir SH, Harris A
Mol Hum Reprod (2018);24(9):433-443
See PubMed abstract

Region-specific microRNA signatures in the human epididymis.
Browne JA, Leir SH, Eggener SE, Harris A
Asian J Androl (2018);:
See PubMed abstract

Molecular characterization of gene regulatory networks in primary human tracheal and bronchial epithelial cells.
Gillen AE, Yang R, Cotton CU, Perez A, Randell SH, Leir SH, Harris A
J Cyst Fibros (2018);17(4):444-453
See PubMed abstract

A transcription factor network represses CFTR gene expression in airway epithelial cells
Mutolo MJ, Leir SH, Fossum SL, Browne JA, Harris A
Biochem J (2018);475(7):1323-1334
See PubMed abstract

The role of microRNAs in chronic respiratory disease: recent insights.
Stolzenburg LR, Harris A
Biol Chem (2018);399(3):219-234
See PubMed abstract

Microvesicle-mediated delivery of miR-1343: impact on markers of fibrosis.
Stolzenburg LR, Harris A
Cell Tissue Res (2018);371(2):325-338
See PubMed abstract

Regulatory dynamics of 11p13 suggest a role for EHF in modifying CF lung disease severity.
Stolzenburg LR, Yang R, Kerschner JL, Fossum S, Xu M, Hoffmann A, Lamar KM, Ghosh S, Wachtel S, Leir SH, Harris A
Nucleic Acids Res (2017);45(15):8773-8784
See PubMed abstract

Differential contribution of cis-regulatory elements to higher order chromatin structure and expression of the CFTR locus.
Yang R, Kerschner JL, Gosalia N, Neems D, Gorsic LK, Safi A, Crawford GE, Kosak ST, Leir SH, Harris A
Nucleic Acids Res (2016);44(7):3082-94
See PubMed abstract

HNF1 regulates critical processes in the human epididymis epithelium.
Browne JA, Yang R, Eggener SE, Leir SH, Harris A
Mol Cell Endocrinol (2016);425:94-102
See PubMed abstract

Hepatocyte nuclear factor 1 coordinates multiple processes in a model of intestinal epithelial cell function.
Yang R, Kerschner JL, Harris A
Biochim Biophys Acta (2016);1859(4):591-8
See PubMed abstract

Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions.
Browne JA, Yang R, Leir SH, Eggener SE, Harris A
Mol Hum Reprod (2016);22(2):69-82
See PubMed abstract

miR-1343 attenuates pathways of fibrosis by targeting the TGF-β receptors.
Stolzenburg LR, Wachtel S, Dang H, Harris A
Biochem J (2016);473(3):245-56
See PubMed abstract

FOXA2 regulates a network of genes involved in critical functions of human intestinal epithelial cells.
Gosalia N, Yang R, Kerschner JL, Harris A
Physiol Genomics (2015);47(7):290-7
See PubMed abstract

Impact of the F508del mutation on ovine CFTR, a Cl- channel with enhanced conductance and ATP-dependent gating.
Cai Z, Palmai-Pallag T, Khuituan P, Mutolo MJ, Boinot C, Liu B, Scott-Ward TS, Callebaut I, Harris A, Sheppard DN
J Physiol (2015);593(11):2427-46
See PubMed abstract

Characterization of primary cultures of adult human epididymis epithelial cells.
Leir SH, Browne JA, Eggener SE, Harris A
Fertil Steril (2015);103(3):647-54.e1
See PubMed abstract

Oxidative stress regulates CFTR gene expression in human airway epithelial cells through a distal antioxidant response element.
Zhang Z, Leir SH, Harris A
Am J Respir Cell Mol Biol (2015);52(3):387-96
See PubMed abstract

Chromatin Dynamics in the Regulation of CFTR Expression.
Gosalia N, Harris A
Genes (Basel) (2015);6(3):543-58
See PubMed abstract

Ets homologous factor regulates pathways controlling response to injury in airway epithelial cells.
Fossum SL, Mutolo MJ, Yang R, Dang H, O'Neal WK, Knowles MR, Leir SH, Harris A
Nucleic Acids Res (2014);42(22):13588-98
See PubMed abstract

Open chromatin mapping identifies transcriptional networks regulating human epididymis epithelial function.
Browne JA, Yang R, Song L, Crawford GE, Leir SH, Harris A
Mol Hum Reprod (2014);20(12):1198-207
See PubMed abstract

Architectural proteins CTCF and cohesin have distinct roles in modulating the higher order structure and expression of the CFTR locus.
Gosalia N, Neems D, Kerschner JL, Kosak ST, Harris A
Nucleic Acids Res (2014);42(15):9612-22
See PubMed abstract

Chromatin remodeling mediated by the FOXA1/A2 transcription factors activates CFTR expression in intestinal epithelial cells.
Kerschner JL, Gosalia N, Leir SH, Harris A
Epigenetics (2014);9(4):557-65
See PubMed abstract

An optimized protocol for isolating primary epithelial cell chromatin for ChIP.
Browne JA, Harris A, Leir SH
PLoS One (2014);9(6):e100099
See PubMed abstract

Ets homologous factor (EHF) has critical roles in epithelial dysfunction in airway disease.
Fossum SL, Mutolo MJ, Tugores A, Ghosh S, Randell SH, Jones LC, Leir SH, Harris A
J Biol Chem (2017);292(26):10938-10949
See PubMed abstract