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Genetics Faculty

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Ann Harris
Professor/Vice Chair of Research
Department of Genetics
School of Medicine
Case Western Reserve University
Biomedical Research Building 724
2109 Adelbert Road
Cleveland, Ohio 44106-4955
Tel: (216) 368-6651
Fax: (216) 368-3432
E-mail: ann.harris@case.edu



About Ann Harris


Research

The main research focus in the laboratory is on transcriptional networks that coordinate chromatin architecture and gene expression in epithelial cells lining the human lung, intestine, pancreas and male genital ducts. These sites are all involved in the common human genetic disease cystic fibrosis (CF). The respiratory epithelium is also compromised in chronic obstructive pulmonary disease (COPD) and asthma. The digestive tract epithelium is a site of active pathology in inflammatory bowel disease (IBD) and other related disorders. Obstruction of the male genital ducts underlies several diseases resulting in male infertility, such as congenital bilateral absence of the vas deferens (CBAVD). Moreover, epithelial cells are the sites of initiation of many types of cancer in the lung, pancreas and intestine. Hence, our work is pertinent to many diseases of epithelia and our research goals are to facilitate new therapeutics for these conditions.


Selected Publications

Kerschner JL, Ghosh S, Paranjapye A, Cosme WR, Audrézet MP, Nakakuki M, Ishiguro H, Férec C, Rommens J, Harris A (2018)
Screening for regulatory variants in 460kb encompassing the CFTR locus in cystic fibrosis patients.
J Mol Diagn;:
See PubMed abstract

Fan Z, Perisse IV, Cotton CU, Regouski M, Meng Q, Domb C, Van Wettere AJ, Wang Z, Harris A, White KL, Polejaeva IA (2018)
A sheep model of cystic fibrosis generated by CRISPR/Cas9 disruption of the CFTR gene.
JCI Insight;3(19):
See PubMed abstract

Browne JA, Leir SH, Eggener SE, Harris A (2018)
Region-specific innate antiviral responses of the human epididymis.
Mol Cell Endocrinol;473:72-78
See PubMed abstract

Yang R, Browne JA, Eggener SE, Leir SH, Harris A (2018)
A novel transcriptional network for the androgen receptor in human epididymis epithelial cells.
Mol Hum Reprod;24(9):433-443
See PubMed abstract

Browne JA, Leir SH, Eggener SE, Harris A (2018)
Region-specific microRNA signatures in the human epididymis.
Asian J Androl;:
See PubMed abstract

Gillen AE, Yang R, Cotton CU, Perez A, Randell SH, Leir SH, Harris A (2018)
Molecular characterization of gene regulatory networks in primary human tracheal and bronchial epithelial cells.
J Cyst Fibros;17(4):444-453
See PubMed abstract

Stolzenburg LR, Harris A (2018)
The role of microRNAs in chronic respiratory disease: recent insights.
Biol Chem;399(3):219-234
See PubMed abstract

Stolzenburg LR, Harris A (2018)
Microvesicle-mediated delivery of miR-1343: impact on markers of fibrosis.
Cell Tissue Res;371(2):325-338
See PubMed abstract

Stolzenburg LR, Yang R, Kerschner JL, Fossum S, Xu M, Hoffmann A, Lamar KM, Ghosh S, Wachtel S, Leir SH, Harris A (2017)
Regulatory dynamics of 11p13 suggest a role for EHF in modifying CF lung disease severity.
Nucleic Acids Res;45(15):8773-8784
See PubMed abstract

Yang R, Kerschner JL, Gosalia N, Neems D, Gorsic LK, Safi A, Crawford GE, Kosak ST, Leir SH, Harris A (2016)
Differential contribution of cis-regulatory elements to higher order chromatin structure and expression of the CFTR locus.
Nucleic Acids Res;44(7):3082-94
See PubMed abstract

Browne JA, Yang R, Eggener SE, Leir SH, Harris A (2016)
HNF1 regulates critical processes in the human epididymis epithelium.
Mol Cell Endocrinol;425:94-102
See PubMed abstract

Yang R, Kerschner JL, Harris A (2016)
Hepatocyte nuclear factor 1 coordinates multiple processes in a model of intestinal epithelial cell function.
Biochim Biophys Acta;1859(4):591-8
See PubMed abstract

Browne JA, Yang R, Leir SH, Eggener SE, Harris A (2016)
Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions.
Mol Hum Reprod;22(2):69-82
See PubMed abstract

Stolzenburg LR, Wachtel S, Dang H, Harris A (2016)
miR-1343 attenuates pathways of fibrosis by targeting the TGF-β receptors.
Biochem J;473(3):245-56
See PubMed abstract

Gosalia N, Yang R, Kerschner JL, Harris A (2015)
FOXA2 regulates a network of genes involved in critical functions of human intestinal epithelial cells.
Physiol Genomics;47(7):290-7
See PubMed abstract

Cai Z, Palmai-Pallag T, Khuituan P, Mutolo MJ, Boinot C, Liu B, Scott-Ward TS, Callebaut I, Harris A, Sheppard DN (2015)
Impact of the F508del mutation on ovine CFTR, a Cl- channel with enhanced conductance and ATP-dependent gating.
J Physiol;593(11):2427-46
See PubMed abstract

Leir SH, Browne JA, Eggener SE, Harris A (2015)
Characterization of primary cultures of adult human epididymis epithelial cells.
Fertil Steril;103(3):647-54.e1
See PubMed abstract

Zhang Z, Leir SH, Harris A (2015)
Oxidative stress regulates CFTR gene expression in human airway epithelial cells through a distal antioxidant response element.
Am J Respir Cell Mol Biol;52(3):387-96
See PubMed abstract

Gosalia N, Harris A (2015)
Chromatin Dynamics in the Regulation of CFTR Expression.
Genes (Basel);6(3):543-58
See PubMed abstract

Fossum SL, Mutolo MJ, Yang R, Dang H, O'Neal WK, Knowles MR, Leir SH, Harris A (2014)
Ets homologous factor regulates pathways controlling response to injury in airway epithelial cells.
Nucleic Acids Res;42(22):13588-98
See PubMed abstract

Browne JA, Yang R, Song L, Crawford GE, Leir SH, Harris A (2014)
Open chromatin mapping identifies transcriptional networks regulating human epididymis epithelial function.
Mol Hum Reprod;20(12):1198-207
See PubMed abstract

Gosalia N, Neems D, Kerschner JL, Kosak ST, Harris A (2014)
Architectural proteins CTCF and cohesin have distinct roles in modulating the higher order structure and expression of the CFTR locus.
Nucleic Acids Res;42(15):9612-22
See PubMed abstract

Kerschner JL, Gosalia N, Leir SH, Harris A (2014)
Chromatin remodeling mediated by the FOXA1/A2 transcription factors activates CFTR expression in intestinal epithelial cells.
Epigenetics;9(4):557-65
See PubMed abstract

Browne JA, Harris A, Leir SH (2014)
An optimized protocol for isolating primary epithelial cell chromatin for ChIP.
PLoS One;9(6):e100099
See PubMed abstract

Fossum SL, Mutolo MJ, Tugores A, Ghosh S, Randell SH, Jones LC, Leir SH, Harris A (2017)
Ets homologous factor (EHF) has critical roles in epithelial dysfunction in airway disease.
J Biol Chem;292(26):10938-10949
See PubMed abstract