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David Buchner
Assistant Professor
Department of Genetics
School of Medicine
Case Western Reserve University
RT400
2109 Adelbert Road
Cleveland, Ohio 44106-4935
Tel: (216) 368-1816
Fax: (216) 368-1835
E-mail: david.buchner@case.edu


About David Buchner

David Buchner received his Ph.D. in Human Genetics from the University of Michigan in Dr. Miriam Meisler's lab where he identified and characterized a modifier gene for neurological disease. He did postdoctoral fellowships in the labs of Dr. David Ginsburg (HHMI, University of Michigan) and Dr. Joe Nadeau (Case Western Reserve University) where he used animal models to identify novel disease genes for bleeding disorders and metabolic disease. He continued studying the pathophysiology of obesity and diabetes while working as an Assistant Research Scientist in Dr. Alan Saltiel's lab in the Life Sciences Institute at the University of Michigan. David joined the faculty at the Case Western Reserve University School of Medicine in 2013 as an Assistant Professor in the Department of Genetics and Genome Sciences with a secondary appointment in the Department of Biochemistry.


Research

Research in Dr. Buchner's laboratory is focused on understanding the genetics and pathophysiology of obesity and diabetes. Although we are in the midst of an obesity epidemic, most genetic susceptibility factors have yet to be discovered. The lab combines both in vivo and in vitro approaches to better understand the mechanisms that influence susceptibility to metabolic disease. There are currently two main projects in the lab. The first is focused on the epigenetic basis of parental and transgenerational effects on offspring disease susceptibility. The second project is focused on the molecular and biochemical regulation of insulin-stimulated glucose uptake by adipoctyes.

Parental and Transgenerational Effects on Obesity and Diabetes

The genes of one's ancestors can have a significant impact on food intake and body weight for multiple generations through a heritable epigenetic mechanism. The Buchner lab is currently utilizing mouse models and next generation sequencing to further explore the relatively unknown genetic and molecular mechanisms that underlie this mode of inheritance.

Regulation of Insulin-Stimulated Glucose Uptake

Hyperglycemia and type 2 diabetes are associated with impaired glucose homeostasis. The major insulin-stimulated glucose transporter Glut4 is a key molecule in the regulation of glucose uptake in adipose tissue as well as in skeletal and cardiac muscle. As the function of Glut4 is impaired in metabolic disease, the lab is interested understanding the regulation of Glut4 expression, and how it is affected in disease.


Selected Publications

Tokunaga M, Inoue M, Jiang Y, Barnes RH, Buchner DA, Chun TH (2014)
Fat depot-specific gene signature and ECM remodeling of Sca1(high) adipose-derived stem cells.
Matrix Biol;:
See PubMed abstract

Cannon MV, Buchner DA, Hester J, Miller H, Sehayek E, Nadeau JH, Serre D (2014)
Maternal nutrition induces pervasive gene expression changes but no detectable DNA methylation differences in the liver of adult offspring.
PLoS One;9(3):e90335
See PubMed abstract

Inoue M, Jiang Y, Barnes RH, Tokunaga M, Martinez-SantibaƱez G, Geletka L, Lumeng CN, Buchner DA, Chun TH (2013)
Thrombospondin 1 mediates high-fat diet-induced muscle fibrosis and insulin resistance in male mice.
Endocrinology;154(12):4548-59
See PubMed abstract

Buchner DA, Geisinger JM, Glazebrook PA, Morgan MG, Spiezio SH, Kaiyala KJ, Schwartz MW, Sakurai T, Furley AJ, Kunze DL, Croniger CM, Nadeau JH (2012)
The juxtaparanodal proteins CNTNAP2 and TAG1 regulate diet-induced obesity.
Mamm Genome;23(7-8):431-42
See PubMed abstract

Yazbek SN, Buchner DA, Geisinger JM, Burrage LC, Spiezio SH, Zentner GE, Hsieh CW, Scacheri PC, Croniger CM, Nadeau JH (2011)
Deep congenic analysis identifies many strong, context-dependent QTLs, one of which, Slc35b4, regulates obesity and glucose homeostasis.
Genome Res;21(7):1065-73
See PubMed abstract

Buchner DA, Yazbek SN, Solinas P, Burrage LC, Morgan MG, Hoppel CL, Nadeau JH (2011)
Increased mitochondrial oxidative phosphorylation in the liver is associated with obesity and insulin resistance.
Obesity (Silver Spring);19(5):917-24
See PubMed abstract

Yazbek SN, Spiezio SH, Nadeau JH, Buchner DA (2010)
Ancestral paternal genotype controls body weight and food intake for multiple generations.
Hum Mol Genet;19(21):4134-44
See PubMed abstract

Hill-Baskin AE, Markiewski MM, Buchner DA, Shao H, DeSantis D, Hsiao G, Subramaniam S, Berger NA, Croniger C, Lambris JD, Nadeau JH (2009)
Diet-induced hepatocellular carcinoma in genetically predisposed mice.
Hum Mol Genet;18(16):2975-88
See PubMed abstract

Buchner DA, Burrage LC, Hill AE, Yazbek SN, O'Brien WE, Croniger CM, Nadeau JH (2008)
Resistance to diet-induced obesity in mice with a single substituted chromosome.
Physiol Genomics;35(1):116-22
See PubMed abstract

Buchner DA, Su F, Yamaoka JS, Kamei M, Shavit JA, Barthel LK, McGee B, Amigo JD, Kim S, Hanosh AW, Jagadeeswaran P, Goldman D, Lawson ND, Raymond PA, Weinstein BM, Ginsburg D, Lyons SE (2007)
pak2a mutations cause cerebral hemorrhage in redhead zebrafish.
Proc Natl Acad Sci U S A;104(35):13996-4001
See PubMed abstract

Buchner DA, Trudeau M, Meisler MH (2003)
SCNM1, a putative RNA splicing factor that modifies disease severity in mice.
Science;301(5635):967-9
See PubMed abstract