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Equipment available in our department includes:  
  • a Leica DM6000 upright microscope that is completely motorized for automated transmitted light (brightfield, darkfield, polarization, and DIC) and automated fluorescence fitted with an 8 filter turret containing filter sets for UV, Blue, Green, Red, G/R, BGR, CFP, YFP, CFP/YFP, and CY5. The Z drive is motorized, providing the conveniences of parfocality, saved focus levels, and automated positioning of various Z levels. The seven lenses on the motorized turret include1.25x, 5x, 10x, 20x, 40x, 63x and 100x. The microscope is equipped with a LCD which displays current settings and offers options for control of all motorized components, and with remote control for Z drive and motorized stage, in addition to programmable buttons. Images are acquired with a Q-Imaging Retiga Xi firewire high speed, 12-bit cooled camera with an IR blocking filter.
  • a Leica DMI6000 inverted microscope that is motorized for automated transmitted light (brightfield and phase contrast) and automated fluorescence filter sets for UV, Blue, Green, Red, G/R, BGR, CFP, YFP, CFP/YFP, and CY5. The Z drive is motorized, providing for parfocality, saved focus levels, and automated positioning of various Z levels. The lenses on the motorized turret include 10x (phase), 20x (phase), 40x (phase), and 63x. Images are acquired with a Hamamatsu Orca-ER digital camera.
  • Both of the above automated and motorized Leica microscopes also have Fluorescence Intensity Manager FIM which allows one to adjust the excitation light and protect specimens, effectively and Excitation Manager which allows one to balance the intensity of multi-label fluorescence.
  • Volocity Acquisition (for acquisition of 3D and 4D volumes) from Improvision, Inc is used for image capture on these two microscopes. Additional Volocity software programs available include Visualization (for visualization and publication), Classification (for measurement and tracking), and Restoration (for deconvolution).
  • All of the lenses on the Leica microscopes in our imaging facility can be interchanged if one needs lower or higher magnification capabilities.
 
  • A Leica SP8 gSTED Super-Resolution Confocal: The system consists of an inverted DMI 6000 adaptive focus microscope with a transmitted brightfield detector, DIC optics, confocal class objectives (10x, 20x, 40x, 60x, 100x), and a motorized super Z galvo stage. The system is equipped with tandem scanners, two PMTs, 3 Hyd SP GaAsP detectors for gated imaging, an 8 channel AOTF and a 5-channel spectrometer. The AOBS system lasers include a 405 nm, Argon (458, 476, 488, 496, 514 nm), a tunable white light (470 to 670 nm), and a 592 nm STED depletion laser. The system is on an air compressor table and is equipped for live cell imaging with a Ludin environmental chamber, heating device and passive gas regulation with connections to CO2 tanks. Software includes Huygens deconvolution, visualization and analysis, dye finder, colocalization, electrophysiology, FLIP, FRET, and FRAP. The workstation is an HPZ600 with Windows 7 professional (64 bit) operating system with a high brilliance 30" LCD flat screen. This gated super-resolution system is capable of resolving to better than 40 nm for fluorophores excited with 434-516 nm wavelengths.
  • a Leica MZFLIII fluorescence stereomicroscope equipped with a SPOT RT slider color digital camera and filters for GFP, dsRed, YFP and CFP.
  • Virtual Microscope: A Leica SCN400 slide scanner for digitizing whole slides in brightfield and fluorescence. Up to 384 slides can be loaded at one time. Magnification to 40x and 3 color RGB fluorescence: (DAPI, GFP, Texas Red filters). Images are automatically uploaded to the "cloud" so you can sit at your own computer to view them. Image analysis software is Visiopharm.
  • Typhoon Phosphorimager: The Typhoon Trio is for fluorescence imaging (488, 532, and 633 nm excitation) of gels, blots, and multiwall plates at resolutions to 25 microns. It is also equipped for radioactive samples using Phophor screen technology. It is not recommended for straight chemiluminescence but fluorescent alternatives such as ECL Plus and Cy-labeled secondary antibodies do allow western blot experiments to be analyzed. Images can be saved as Tiff files and analysis is performed using ImageQuant software on the Typhoon computer
For More Information Please Contact Patty Conrad at 368-0199  
Last update on: 30th September 2015