Imaging and Training Facility
The Genetics Imaging Facility is a light microscope facility dedicated to bringing state-of-the-art methods in modern imaging to biomedical and research scientists at Case Western Reserve University. The Imaging Facility consists of a Leica TCS SP8 confocal equipped for gated STED super resolution to 40 nm, 2 Leica fluorescence stereomicroscopes, 3 Leica widefield microscopes, a Leica SCN 400 slide scanner capable of bulk loading 384 slides for bright field and fluorescence, a GE Typhoon Trio for chemiluminescence and fluorescence imaging of gels and blots, and a Perkin Elmer Operetta high throughput analysis system. The lasers on the confocal are 405 nm, Argon (458, 476, 488, 496, 514 nm) and a tunable white light laser covering 470 through 670 nm excitation. The confocal and one of the widefield microscopes have environmental chambers for live cell imaging. All of the widefield microscopes have CCD cameras, two of which are color cameras to aid in histological analysis. Objective lenses are from 1.5x through 100x with optics for brightfield, darkfield, phase, polarization, DIC, Hoffman Modulation contrast, and fluorescence. Remote workstations are available for deconvolution, image processing, analysis and stereology.