DNA Sequencing Core
Located on the 7th floor of the BRB, the Genomics Core Facility provides sequencing and genotyping capabilities that is available to all investigators at Case. Custom designed SNP projects as well as human and mouse linkage analysis projects can also be handled by the Core.
The Core is equipped with an ABI Prism 3730 DNA Analyzer which handles the majority of all sequencing samples and an ABI Prism 3130 Analyzer for genotyping samples. The Illumina Beadstation 500GX Genetic Analysis System runs all custom designed SNP projects and linkage projects using BeadArray technology on two different Array of ArraysTM formats, the Sentrix® Array Matrix and the Sentrix® BeadChip. Large-scale genome-wide SNP association studies are performed using Illumina's Infinium assay.
The mission of the Core is to provide better data at a reduced cost and turn-around time with respect to outside sources.
Genetics and Genome Sciences Imaging Facility
The Imaging Facility is a light microscope facility dedicated to bringing state-of-the-art methods in modern imaging to our biomedical and research scientists here at CASE. The Imaging Facility consists of nine complete microscope workstations (6 widefield, 2 of which are completely motorized and automated; 1 widefield equipped for live-cell imaging and deconvolution; 1 confocal equipped for live-cell imaging; and 1 fluorescence stereomicroscope). All of the widefield microscopes are equipped with CCD cameras, two of which are color cameras to aid in histological analysis. Lenses are from 1.5x through 100x, with optics for Brightfield, Darkfield, Phase, Polarization, DIC, Hoffman Modulation Contrast, and Fluorescence. The lasers on the confocal are 405 nm, Argon (458, 476, 488, 496, 514 nm), Green HeNe (543 nm), Orange HeNe (594 nm), and Red HeNe (633 nm). There is also a remote workstation for analysis. Software processing programs include those for visualization (2-D and 3-D), publication, measurement and tracking, and deconvolution.
The Department of Genetics and Genome Sciences Imaging Facility is located on the 7th floor of the Biomedical Research Building. For further information go to our Imaging Facility site.
Shared Instrumentation Grants from NIH NCRR (numbers RR-017980 and RR-021228) allowed us to purchase our Leica TCS SP2 AOBS filter free UV/spectral confocal laser scanning microscope and the two Leica widefield automated and motorized microscopes. Departmental funds allowed us to purchase the Leica multi-dimensional workstation for live-cell imaging. HHMI provided the funds for the GFP stereomicroscope and two of the widefield setups.
Pluripotent Stem Cell Facility
The Pluripotent Stem Cell Facility is a fee-for-service core facility dedicated to the growth, derivation, and differentiation of pluripotent stem cells. Services include derivation of human induced pluripotent stem cell lines (iPS cells), derivation of mouse embryonic stem cell lines, training in pluripotent stem cell culture (ES and iPS), consultation on pluripotent stem cell-based experiments, and access to validated pluripotent stem cell reagents including MEFs.
Case Transgenic and Targeting Core
The Case Transgenic and Targeting Facility provides transgenic, targeting (knockouts, knockins, targe ted point mutations, conditional knockouts), chimera, rederivation and cryopreservation services for the Cleveland Biomedical Research Community. The Facility Director is Ronald Conlon of the Department of Genetics and Genome Sciences.
The Case Transgenic and Targeting Facility office is located in Wolstein Research Bldg 2521.
Services are contracted on a fee-for-service, first-come-first-serve basis with an oustanding record of success.
For more information please go to http://ko.cwru.edu