Zhenghe Wang
Assistant Professor
Ph.D. Training Faculty
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Department of Genetics
School of Medicine
Case Western Reserve University
Wolstein Research Building
2103 Cornell Road
Cleveland, Ohio 44106
Tel: (216) 368-0446
Fax: (216) 368-3432
E-mail: zhenghe.wang@case.edu
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About Zhenghe Wang
John Wang received his Ph.D. in Microbiology from University of Virginia in 2001 for work on the molecular mechanism of sister chromatid cohesion in yeast. He was a postdoctoral fellow with Dr. Bert Vogelstein in the Sydney Kimmel Comprehensive Cancer Center at Johns Hopkins University, where he used high throughput sequence-based mutational analysis to identify somatic mutations in human colorectal cancer. He also dissected the APC/β-catenin pathways in colorectal cells using somatic cell knockout technique. Dr. Wang joined the Department of Genetics and the Case Comprehensive Cancer Center in August, 2005.
Research
Dr. Wang and his research group are interested in dissecting the signaling pathways controlled by phosphatases that are mutated in cancer. The role of phosphatases in tumorigenesis is less well-defined than that of kinases. However, increasing evidence suggests that phosphatases are an important component of tumor development. We have recently identified six protein tyrosine phosphatase genes mutated in colorectal cancers using high throughput sequence-based mutational analysis. The majority of mutations in phosphatases characterized in colon cancers clearly cause inactivation of these genes suggesting that these phosphatases function as tumor suppressor genes in normal colon cells. We are currently studying the signal pathways regulated by the two frequently mutated phosphatases, PTPRT and PTPN13, using genetic and biochemical approaches to determine how these genes prevent cancer development.
These studies are part of our overall laboratory program focused on identifying novel genetic alterations, such as somatic mutations, gene amplifications and deletions, which alter critical gene functions involved in development of colon and gastric cancers.
Selected Publications
| Zhao Y, Zhang X, Guda K, Lawrence E, Sun Q, Watanabe T, Iwakura Y, Asano M, Wei L, Yang Z, Zheng W, Dawson D, Willis J, Markowitz SD, Satake M, Wang Z (2010) | | Identification and functional characterization of paxillin as a target of protein tyrosine phosphatase receptor T. | | Proc Natl Acad Sci U S A;: | | See PubMed abstract |
| Wang P, Zou F, Zhang X, Li H, Dulak A, Tomko RJ, Lazo JS, Wang Z, Zhang L, Yu J (2009) | | microRNA-21 Negatively Regulates Cdc25A and Cell Cycle Progression in Colon Cancer Cells. | | Cancer Res;: | | See PubMed abstract |
| Queiroz de Oliveira PE, Zhang L, Wang Z, Lazo JS (2009) | | Hypoxia-mediated regulation of Cdc25A phosphatase by p21 and miR-21. | | Cell Cycle;8(19): | | See PubMed abstract |
| Schnetz MP, Bartels CF, Shastri K, Balasubramanian D, Zentner GE, Balaji R, Zhang X, Song L, Wang Z, Laframboise T, Crawford GE, Scacheri PC (2009) | | Genomic distribution of CHD7 on chromatin tracks H3K4 methylation patterns. | | Genome Res;19(4):590-601 | | See PubMed abstract |
| Wang Z (2009) | | Epitope tagging of endogenous proteins for genome-wide chromatin immunoprecipitation analysis. | | Methods Mol Biol;567:87-98 | | See PubMed abstract |
| Guo C, Zhang X, Fink SP, Platzer P, Wilson K, Willson JK, Wang Z, Markowitz SD (2008) | | Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA glycosylase. | | Cancer Res;68(15):6118-26 | | See PubMed abstract |
| Yu J, Becka S, Zhang P, Zhang X, Brady-Kalnay SM, Wang Z (2008) | | Tumor-Derived Extracellular Mutations of PTPRT/PTP{rho} Are Defective in Cell Adhesion. | | Mol Cancer Res;6(7):1106-13 | | See PubMed abstract |
| Wei L, Jamonnak N, Choy J, Wang Z, Zheng W (2008) | | Differential binding modes of the bromodomains of CREB-binding protein (CBP) and p300 with acetylated MyoD. | | Biochem Biophys Res Commun;368(2):279-84 | | See PubMed abstract |
| Zhang X, Guo C, Chen Y, Shulha HP, Schnetz MP, Laframboise T, Bartels CF, Markowitz S, Weng Z, Scacheri PC, Wang Z (2008) | | Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies. | | Nat Methods;5(2):163 -165 | | See PubMed abstract |
| Zhang X, Guo A, Yu J, Possemato A, Chen Y, Zheng W, Polakiewicz RD, Kinzler KW, Vogelstein B, Velculescu VE, Wang ZJ (2007) | | Identification of STAT3 as a substrate of receptor protein tyrosine phosphatase T. | | Proc Natl Acad Sci U S A;104(10):4060-4 | | See PubMed abstract |
| Ma PC, Zhang X, Wang ZJ (2006) | | High-Throughput Mutational Analysis of Human Cancer Genome | | Pharmacogenomics;7:597-612 | | See PubMed abstract |
| Lengauer C and Wang Z.
(2004) | | From spindle checkpoint to cancer. | | Nature Genetics;36(11):1144-5 | | See PubMed abstract |
| Wang Z, Shen D, Parsons W, Bardelli A, Sager J, Szabo S, Ptak J, Silliman N, Peters BA, van der Heijden MS, Parmigiani G, Yan H, Wang TL, Riggins G, Powell S, M,James, Willson KV, Markowitz S, Kinzler KW, Vogelstein B, and Velculescu VE.
(2004) | | Mutational analysis of the tyrosine phosphatome in colorectal cancers. | | Science;304(5674):1164-6 | | See PubMed abstract |
| Wang Z, Cummins JM, Shen D, Cahill DP, Jallepalli PV, Wang TL, Parsons DW, Traverso G, Awad M, Silliman N, Ptak J, Szabo S, Willson JK, Markowitz SD, Goldberg ML, Karess R, Kinzler KW, Vogelstein B, Velculescu VE, and Lengauer C. (2004) | | Three classes of genes mutated in colorectal cancers with chromosomal instability. | | Cancer Research;64(9):2998-3001 | | See PubMed abstract |
| Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, Gazdar A, Powell SM, Riggins GJ, Willson JK, Markowitz S, Kinzler KW, Vogelstein B, Velculescu VE.
(2004) | | High frequency of mutations of the PIK3CA gene in human cancers. | | Science;304(5670):554 | | See PubMed abstract |
| Wang Z, Vogelstein B, and Kinzler KW (2003) | | Phosphorylation of ß-Catenin at S33, S37, or T41 can occur in the absence of phosphorylation at S45 in Colon Cancer Cells. | | Cancer Research;63(17):5234-5 | | See PubMed abstract |
| Yu J, Wang Z, Kinzler KW, Vogelstein B, and Zhang L.
(2003) | | PUMA mediates the apoptotic response to p53 in colorectal cancer cells. | | Proc Natl Acad Sci U S A;100(4):1931-6 | | See PubMed abstract |
| Chan TA, Wang Z, Dang L, Vogelstein B, and Kinzler KW.
(2002) | | Targeted inactivation of CTNNB1 reveals unexpected effects of ß-catenin mutation. | | Proc Natl Acad Sci U S A.;99(12):8265-70 | | See PubMed abstract |
| Wang Z, Castano IB, De Las Penas A, Adams C, and Christman MF.
(2000) | | Pol kappa: A DNA polymerase required for sister chromatid cohesion | | Science;289(5480):774-9 | | See PubMed abstract |
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