Peter Scacheri
Assistant Professor
Ph.D. Training Faculty
|
|
Department of Genetics
School of Medicine
Case Western Reserve University
Biomedical Research Building 627
2109 Adelbert Road
Cleveland, Ohio 44106-4955
Tel: (216) 368-3458
Fax: (216) 368-3432
E-mail: peter.scacheri@case.edu
|
About Peter Scacheri
Peter Scacheri graduated with a BS in Biology from Gettysburg College and earned his Ph.D. in Biochemistry and Molecular Genetics from the University of Pittsburgh. His graduate work was focused on the molecular mechanisms underlying dominantly inherited forms of muscular dystrophy. Peter was awarded an American Heart Association Graduate Student Award for his work on oculopharyngeal muscular dystrophy. His postdoctoral fellowship was in the lab of Francis Collins at the National Human Genome Research Institute at the National Institutes of Health. There he combined mouse genetics and genomics to study a type of cancer that affects the endocrine organs. Peter won several awards during his postdoctoral fellowship, including the NHGRI Intramural Research Award for outstanding scientific achievement. In addition, Peter is committed to education in genetics, from the high school level to medical students and graduate students. He participated in the NHGRI's DNA Day and taught cell biology and genetics courses in the biotechnology graduate program of Johns Hopkins University. He was a volunteer at the Muscular Dystrophy Association's summer camp for several years. An avid cyclist and dedicated scientist, Peter was one of 26 individuals chosen to be on the National Team of the Bristol Myers Squibb Tour of Hope in 2003. The inaugural Tour of Hope was designed to raise awareness about cancer clinical trials, combining educational events with Lance Armstrong with a relay-style cross-country cycling tour. Peter joined the Department of Genetics in 2006.
Research
The Scacheri lab utilizes cutting-edge genomics technologies to investigate mechanisms of human genetic diseases. Currently, the lab is focused on CHARGE syndrome, a genetic condition characterized by a complex constellation of birth defects. De novo mutations in the gene encoding chromodomain helicase DNA binding protein 7 (CHD7) are the major cause of CHARGE syndrome. The Scacheri lab recently discovered that CHD7 regulates transcription by targeting active gene enhancer elements. These findings suggest that the multiple birth defects in CHARGE syndrome are a result of dysregulated transcription during development. In addition to the work on CHD7 and CHARGE syndrome, the Scacheri lab is using colon cancer as a paradigm to study epigenetic mechanisms of gene silencing and activation in cancer. His lab is comprised of two research technicians, two postdoctoral fellows, and four graduate students.
Selected Publications
| Tie F, Banerjee R, Stratton CA, Prasad-Sinha J, Stepanik V, Zlobin A, Diaz MO, Scacheri PC, Harte PJ (2009) | | CBP-mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing. | | Development;136(18):3131-41 | | See PubMed abstract |
| Tao M, Scacheri PC, Marinis JM, Harhaj EW, Matesic LE, Abbott DW (2009) | | ITCH K63-ubiquitinates the NOD2 binding protein, RIP2, to influence inflammatory signaling pathways. | | Curr Biol;19(15):1255-63 | | See PubMed abstract |
| Schnetz MP, Bartels CF, Shastri K, Balasubramanian D, Zentner GE, Balaji R, Zhang X, Song L, Wang Z, Laframboise T, Crawford GE, Scacheri PC (2009) | | Genomic distribution of CHD7 on chromatin tracks H3K4 methylation patterns. | | Genome Res;: | | See PubMed abstract |
| Johnson DS, Li W, Gordon DB, Bhattacharjee A, Curry B, Ghosh J, Brizuela L, Carroll JS, Brown M, Flicek P, Koch CM, Dunham I, Bieda M, Xu X, Farnham PJ, Kapranov P, Nix DA, Gingeras TR, Zhang X, Holster H, Jiang N, Green RD, Song JS, McCuine SA, Anton E, Nguyen L, Trinklein ND, Ye Z, Ching K, Hawkins D, Ren B, Scacheri PC, Rozowsky J, Karpikov A, Euskirchen G, Weissman S, Gerstein M, Snyder M, Yang A, Moqtaderi Z, Hirsch H, Shulha HP, Fu Y, Weng Z, Struhl K, Myers RM, Lieb JD, Liu XS (2008) | | Systematic evaluation of variability in ChIP-chip experiments using predefined DNA targets. | | Genome Res;18(3):393-403 | | See PubMed abstract |
| Zhang X, Guo C, Chen Y, Shulha HP, Schnetz MP, LaFramboise T, Bartels CF, Markowitz S, Weng Z, Scacheri PC, Wang Z (2008) | | Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies. | | Nat Methods;5(2):163-5 | | See PubMed abstract |
| Agarwal SK, Impey S, McWeeney S, Scacheri PC, Collins FS, Goodman RH, Spiegel AM, Marx SJ (2007) | | Distribution of menin-occupied regions in chromatin specifies a broad role of menin in transcriptional regulation. | | Neoplasia;9(2):101-7 | | See PubMed abstract |
| Crawford GE, Davis S,Scacheri PC,Renaud G, Halawi MJ, Erdos MR, Green R, Meltzer PS, Wolfsberg TG, Collins FS. (2006) | | DNase-chip: A high resolution method to identify DNaseI hypersensitive sites using tiled microarrays, | | Nature Methods;3:503-9 | | See PubMed abstract |
| Scacheri PC, Davis S, Odom DT, Crawford GE, Perkins S, Halawi MJ, Agarwal SK, Marx SJ, Spiegel AM, Meltzer PS, Collins FS. (2006) | | Genome-wide analysis of menin binding provides insights into MEN1 tumorigenesis. | | PLoS Genet.;2(4):e51 | | See PubMed abstract |
| Scacheri PC, Crawford GE, Davis S. (2006) | | Statistics for ChIP-chip and DNAse hypersensitivity experiments on NimbleGen arrays. | | Methods Enzym.;411:270-82 | | See PubMed abstract |
| Agarwal SK, Kennedy PA, Scacheri PC, Novotny EA, Hickman AB, Cerrato A, Rice TS, Moore JB, Rao S, Ji Y, Mateo C, Libutti SK, Oliver B, Chandrasekharappa SC, Burns AL, Collins FS, Spiegel AM, Marx SJ. (2005) | | Menin molecular interactions: insights into normal functions and tumorigenesis. | | Horm Metab Res.;37(6):369-74 | | See PubMed abstract |
| Scacheri PC, Crabtree JS, Kennedy AL, Swain GP, Ward JM, Marx SJ, Spiegel AM, Collins FS. (2004) | | Homozygous loss of menin is well tolerated in liver, a tissue not affected in MEN1. | | Mamm Genome.;15(11):872-7 | | See PubMed abstract |
| Scacheri PC, Kennedy AL, Chin K, Miller MT, Hodgson JG, Gray JW, Marx SJ, Spiegel AM, Collins FS. (2004) | | Pancreatic insulinomas in multiple endocrine neoplasia, type I knockout mice can develop in the absence of chromosome instability or microsatellite instability. | | Cancer Res.;64(19):7039-44 | | See PubMed abstract |
| Agarwal SK, Lee Burns A, Sukhodolets KE, Kennedy PA, Obungu VH, Hickman AB, Mullendore ME, Whitten I, Skarulis MC, Simonds WF, Mateo C, Crabtree JS, Scacheri PC, Ji Y, Novotny EA, Garrett-Beal L, Ward JM, Libutti SK, Richard Alexander H, Cerrato A, Parisi MJ, Santa Anna-A S, Oliver B, Chandrasekharappa SC, Collins FS, Spiegel AM, Marx SJ. (2004) | | Molecular pathology of the MEN1 gene. | | Ann N Y Acad Sci.;1014:189-98 | | See PubMed abstract |
| Scacheri PC, Rozenblatt-Rosen O, Caplen NJ, Wolfsberg TG, Umayam L, Lee JC, Hughes CM, Shanmugam KS, Bhattacharjee A, Meyerson M, Collins FS. (2004) | | Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells. | | Proc Natl Acad Sci U S A.;101(7):1892-7 | | See PubMed abstract |
| Crabtree JS, Scacheri PC, Ward JM, McNally SR, Swain GP, Montagna C, Hager JH, Hanahan D, Edlund H, Magnuson MA, Garrett-Beal L, Burns AL, Ried T, Chandrasekharappa SC, Marx SJ, Spiegel AM, Collins FS. (2003) | | Of mice and MEN1: Insulinomas in a conditional mouse knockout. | | Mol Cell Biol.;23(17):6075-85 | | See PubMed abstract |
| Yim SH, Ward JM, Dragan Y, Yamada A, Scacheri PC, Kimura S, Gonzalez FJ. (2003) | | Microarray analysis using amplified mRNA from laser capture microdissection of microscopic hepatocellular precancerous lesions and frozen hepatocellular carcinomas reveals unique and consistent gene e | | Toxicol Pathol.;31(3):295-303 | | See PubMed abstract |
| Scacheri PC, Gillanders EM, Subramony SH, Vedanarayanan V, Crowe CA, Thakore N, Bingler M, Hoffman EP. (2002) | | Novel mutations in collagen VI genes: expansion of the Bethlem myopathy phenotype. | | Neurology.;58(4):593-602 | | See PubMed abstract |
| Scacheri PC, Crabtree JS, Novotny EA, Garrett-Beal L, Chen A, Edgemon KA, Marx SJ, Spiegel AM, Chandrasekharappa SC, Collins FS.
(2001) | | Bidirectional transcriptional activity of PGK-neomycin and unexpected embryonic lethality in heterozygote chimeric knockout mice. | | Genesis.;30(4):259-63 | | See PubMed abstract |
| Crabtree JS, Scacheri PC, Ward JM, Garrett-Beal L, Emmert-Buck MR, Edgemon KA, Lorang D, Libutti SK, Chandrasekharappa SC, Marx SJ, Spiegel AM, Collins FS. (2001) | | A mouse model of multiple endocrine neoplasia, type 1, develops multiple endocrine tumors. | | Proc Natl Acad Sci U S A.;98(3):1118-23 | | See PubMed abstract |
| Scacheri PC, Hoffman EP, Fratkin JD, Semino-Mora C, Senchak A, Davis MR, Laing NG, Vedanarayanan V, Subramony SH. (2000) | | A novel ryanodine receptor gene mutation causing both cores and rods in congenital myopathy. | | Neurology.;12;55(11):1689-96. | | See PubMed abstract |
| Scacheri PC, Garcia C, Hebert R, Hoffman EP. (1999) | | Unique PABP2 mutations in "Cajuns" suggest multiple founders of oculopharyngeal muscular dystrophy in populations with French ancestry. | | Am J Med Genet.;86(5):477-81 | | See PubMed abstract |
| James SW, Bullock KA, Gygax SE, Kraynack BA, Matura RA, MacLeod JA, McNeal KK, Prasauckas KA, Scacheri PC, Shenefiel HL, Tobin HM, Wade SD.
(1999) | | nimO, an Aspergillus gene related to budding yeast Dbf4, is required for DNA synthesis and mitotic checkpoint control. | | J Cell Sci.;112 ( Pt 9):1313-24. | | See PubMed abstract |
| Morrone A, Zammarchi E, Scacheri PC, Donati MA, Hoop RC, Servidei S, Galluzzi G, Hoffman EP. (1997) | | Asymptomatic dystrophinopathy. | | Am J Med Genet.;69(3):261-7. | | See PubMed abstract |
| Garcia-Heras J, Martin JA, Witchel SF, Scacheri PC. (1997) | | De novo der(X)t(X;10)(q26;q21) with features of distal trisomy 10q: case report of paternal origin identified by late replication with BrdU and the human androgen receptor assay (HAR). | | J Med Genet;34: 242-245 | |
| Hoffman EP, Pegoraro E, Scacheri PC, Burns RG, Taber JW, Weiss L, Spiro A, and Blattner P. (1996) | | Genetic counseling of isolated carriers of Duchenne muscular dystrophy. | | Am J Med Genet;63: 573-580 | |
| James SW, Mirabito PM, Scacheri PC, Morris NR. (1995) | | The Aspergillus nidulans bimE (blocked-in-mitosis) gene encodes multiple cell cycle functions involved in mitotic checkpoint control and mitosis. | | J Cell Sci.;108 ( Pt 11):3485-99 | | See PubMed abstract |
|
